FIU Robert Stempel College of Public Health and Social Work

Florida International University - Robert Stempel College of Public Health and Social Work
Florida International University - Robert Stempel College of Public Health and Social Work
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Available Ph.D. Project - Cooke

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Marcus S. Cooke, Ph.D., F.R.C.Path.
Professor of Environmental & Occupational Health

Genetic and Epigenetic Effects of Oxidative Stress: a Role in Disease

Both endogenous and exogenous processes generate free radicals and reactive oxygen species. If this production overwhelms the cell’s antioxidant defenses, this leads to oxidative stress. Oxidative stress has an important role in numerous pathological conditions, including cancer, cardiovascular, respiratory and neurological disease via its detrimental effects upon cellular function. This includes mutation, altered gene expression, acceleration of telomere shortening and the promotion of microsatellite instability. Damage to DNA is receiving most attention, due to the significant effects upon cell function, plus clear mechanistic relevance to the disease process.

The aim of my research is to better understand how oxidative stress alters cellular function, and how this then leads to disease. This is achieved by a combination of molecular biology techniques, and biochemical analyses applied to both cell culture, and studies in humans.

A better understanding of how oxidative stress influences disease, will allow us develop intervention strategies, plus the development of biomarkers of oxidative stress may have predictive and prognostic properties.

References 

  1. Cooke, MS., Evans, MD., Dizdaroglu, M. and Lunec, J. (2003) Oxidative DNA damage: mechanisms, mutation and disease. FASEB J., 17, 1195-1214.
  2. Evans, MD. and Cooke, MS. (2004) Factors contributing to the outcome of oxidative damage to nucleic acids. Bioessays. 26, #5, 533-542.
  3. Evans, MD., Saparbaev, M. and Cooke, MS. (2010) DNA repair and the origins of urinary oxidized 2'-deoxyribonucleosides. Mutagenesis 25, 433-442.
  4. Al-Salmani, K. et al. (2011) Evaluation of storage and DNA damage analysis of whole blood by Comet assay. Free Radic. Biol. Med., 51, 719-725.
  5. Barregard, L. et al. (2013) Human and methodological sources of variability in the measurement of urinary 8-oxo-7,8-dihydro-2’-deoxyguanosine. Antioxidants and Redox Signaling 18, 2377-2391.

How to Apply

Applications are accepted through FindAPhD.

CONTACT US

Department of Environmental & Occupational Health
Robert Stempel College of Public Health & Social Work
Florida International University
Modesto A. Maidique Campus, AHC5 355
11200 SW 8th Street
Miami, FL 33199
Phone: 305-348-7778
Fax: 305-348-1996

Marcus S. Cooke, Ph.D.
Professor and Chair
305-348-7778
mcooke@fiu.edu