Oxidative Stress Group

Name of Lab:

Oxidative Stress Group

Department:

Environmental Health Sciences

Subject area description:

The lab focuses on oxidative stress and genomic instability, from basic mechanisms to translational application of validated biomarkers. It concentrates on the formation and repair of damage to nuclear/mitochondrial DNA, the nucleotide precursor pools and DNA adductomics such as: (i) the totality of adducts in cellular DNA and urine; and (ii) the genome-wide mapping of damage.

Lab Illustration

Environmental agents can cause DNA damage that is linked to major human diseases.

Exposure to stressors in the environment and normal processes in our bodies can lead to the formation of reactive chemicals such as reactive oxygen species (ROS) and reactive nitrogen species (RNS). These chemicals can damage cellular molecules, such as DNA and RNA and affect cell function. They are implicated in neurodegenerative and cardiovascular diseases, cancer and aging. Protecting the cell are antioxidant defenses and DNA repair, which either intercept ROS or repair the DNA damage after it is formed.

Techniques/assays:

In vitro

  • Nuclear DNA base excision repair
  • Nuclear DNA nucleotide excision repair
  • Formation and repair of nuclear DNA Inter-strand crosslinks
  • Cellular antioxidant capacity
  • Mitochondrial DNA damage and repair
  • Nuclear and mitochondrial DNA adductomics for the genome-wide mapping of damage and repair

In/ex vivo

  • Nuclear DNA damage in peripheral blood mononuclear cells (pin prick of blood)
  • Biomarkers of oxidative stress in extracellular matrices e.g. urine, serum or plasma:
    • 8-oxo-7,8-dihydroguanosine – derived from RNA/GTP pool
    • 8-oxo-7,8-dihydro-2’-deoxyguanosine – derived from DNA/dGTP pool

Publications:

http://www.ncbi.nlm.nih.gov/sites/myncbi/1VgOxZHwqORki/bibliography/47226589/public/?sort=date&direction=ascending

Principal Investigator:

Dr. Marcus S. Cooke

Members:

Dr. Mahsa Karbaschi, postdoctoral fellow

Hadi Abdulwahed, doctoral candidate

Yunhee Ji, doctoral candidate

Yenny Diaz, doctoral candidate

Jesenia Perez, MARC U-STAR student

Jessica Cobb, undergraduate student

Visiting Scientists:

Dr. Mu-Rong Chao (Chung Shan Medical University, Taiwan)

Dr. Chiung Hu (Chung Shan Medical University, Taiwan)

Dr. John Anetor (University of Ibadan, Nigeria)

Dr. Gloria Anetor (University of Ibadan, Nigeria)