Manivannan Yegambaram

Focus

Post-translational modifications of proteins associated with Pulmonary hypertension and acute lung injury; Metabolic reprogramming in Pulmonary hypertension

Biography

Dr. Manivannan Yegambaram is a Research Assistant Professor at Florida International University’s Center for Translational Science and is part of the Department of Environmental Health Sciences at Stempel College. He earned his bachelor’s degree in Zoology from Fergusson College and a master’s degree in Health Sciences from University of Pune in India. Later, he received a Victoria University of Wellington Doctoral Scholarship and completed his Ph.D. degree in Cell and Molecular Biology from Victoria University of Wellington in New Zealand. His Ph.D. research under the mentorship of Prof. Paul Atkinson focused on localizing misfolded glycoproteins in the cells arising from a disorder of glycosylation using the Perkin Elmer Evotec Opera high content screening microscope.

After obtaining his Ph.D. degree, Dr. Yegambaram moved to the United States and held two postdoctoral fellowships. First, he worked with Prof. Rolf Halden at the Arizona State University and established the Proteomics and Mass Spectrometry recharge facility. Dr. Yegambaram focused on the assessment of bioaccumulative and toxic organic environmental pollutants in tissue of Alzheimer's disease patients. Utilizing LC-MS, GC-MS and MALDI-TOF mass spectrometry, he identified organ-specific human body burdens to select environmental pollutants/toxicants suspected to constitute Alzheimer's disease risk factors. Later, he joined the College of Medicine at University of Arizona as a postdoctoral research associate and worked with Prof. Stephen Black to focus on Pulmonary hypertension and acute lung injury. Using MALDI-TOF mass spectrometry he identified post-translational modification signature profiles reflective of Pulmonary hypertension and acute lung injury. In 2021, he joined the Center for Translational Science at Florida International University as a faculty member.

Research interests

Proteomics, Mass spectrometry, Metabolomics, Microscopy, High throughput drug screening, Cell and Molecular Biology

Publications

Shuxin Liang, Manivannan Yegambaram, Ting Wang, Jian Wang, Stephen M Black, Haiyang Tang. Mitochondrial Metabolism, Redox, and Calcium Homeostasis in Pulmonary Arterial Hypertension. Biomedicines. 2022 Feb 1;10(2):341. doi: 10.3390/biomedicines10020341.

Ting Wang*, Manivannan Yegambaram*, Christine Gross, Xutong Sun, Qing Lu, Hui Wang, Xiaomin Wu, Haiyang Tang, Saurabh Aggarwal, and Stephen Black. Rac1 nitration at y32 is involved in the endothelial barrier disruption associated with lipopolysaccharide-mediated acute lung injury. Redox Biology. 2021 Jan; 38:101794. doi: 10.1016/j.redox.2020.101794. Epub 2020 Nov 13.

* These authors contributed equally to this work.

Evgeny A Zemskov, Xiaomin Wu, Saurabh Aggarwal, Manivannan Yegambaram, Christine Gross, Qing Lu, Hui Wang, Haiyang Tang, Ting Wang, Stephen M Black. Nitration of protein kinase G-Iα modulates cyclic nucleotide crosstalk via phosphodiesterase 3A: Implications for acute lung injury. J Biol Chem. 2021 Aug;297(2):100946. doi: 10.1016/j.jbc.2021.100946. Epub 2021 Jul 10.

Xiaomin Wu, Xutong Sun, Shruti Sharma, Qing Lu, Manivannan Yegambaram, Yali Hou, Ting Wang, Jeffrey R. Fineman, and Stephen Black. Arginine recycling in endothelial cells is regulated by hsp90 and the ubiquitin proteasome system. Nitric Oxide. 2021 Mar 1;108:12-19. doi: 10.1016/j.niox.2020.12.003.

Hui Wang, Xutong Sun, Qing Lu, Evgeny A Zemskov, Manivannan Yegambaram, Xiaomin Wu, Ting Wang, Haiyang Tang, and Stephen M Black. The mitochondrial redistribution of eNOS is involved in lipopolysaccharide induced inflammasome activation during acute lung injury. Redox Biology. 2021 Jan 26;41:101878. doi: 10.1016/j.redox.2021.101878.

Qing Lu, Evgeny A. Zemskov, Xutong Sun, Hui Wang, Manivannan Yegambaram, Xiaomin Wu, Alejandro Garcia-Flores, Shanshan Song, Haiyang Tang, Archana Kangath, Gabriela Zubiate Cabanillas, Jason X.-J. Yuan, Ting Wang, Jeffrey R. Fineman, and Stephen Black. Activation of the mechanosensitive ca2+ channel trpv4 induces endothelial barrier permeability via the disruption of Mitochondrial bioenergetics. Redox Biology. 2021 Jan;38:101785. doi: 10.1016/j.redox.2020.101785. Epub 2020 Nov 12.

Xutong Sun, Qing Lu, Manivannan Yegambaram, Sanjiv Kumar, Ning Qu, Anup Srivastava, Ting Wang, Jeffrey R. Fineman, and Stephen M. Black. TGF-β1 attenuates mitochondrial bioenergetics in pulmonary arterial endothelial cells via the disruption of carnitine homeostasis. Redox Biol. 2020 Sep; 36: 101593. PMID: 32554303

Wojciech Ornatowski, Qing Lu, Manivannan Yegambaram, Alejandro E Garcia, Evgeny A Zemskov, Emin Maltepe, Jeffrey R Fineman, Ting Wang,and Stephen M Black. Complex interplay between autophagy and oxidative stress in the development of pulmonary disease. Redox Biol. 2020 Sep;36:101679. doi: 10.1016/j.redox.2020.101679. Epub 2020 Aug 11

Bhagyashree Manivannan*, Manivannan Yegambaram*, Samuel Supowit, Thomas. Beach and Rolf Halden. Assessment of persistent, bioaccumulative and toxic organic environmental pollutants in liver and adipose tissue of Alzheimer's disease patients and age- matched controls. Current Alzheimer Research. 2019. doi: 10.2174/1567205016666191010114744. PMID: 31660829

These authors contributed equally to this work.

 Manivannan Yegambaram, Bhagyashree Manivannan, Thomas Beach and Rolf U. Halden. Role of Environmental Contaminants in the Etiology of Alzheimer’s Disease. Current Alzheimer Research. 2015. 12, 116-146. PMID: 25654508.

Peter W. Bircham, David R. Maass, Christina A. Roberts, Poh Y. Kiew, Yee S. Low, Manivannan Yegambaram, James Matthews, Cameron A. Jack and Paul H. Atkinson. Secretory pathway genes assessed by high-throughput microscopy and synthetic genetic array analysis. Molecular BioSystems. 2011. 7, 2589-2598. PMID: 21731954.