Professor, Department of Environmental Health Sciences
Oxidative Stress and Genomic Instability
Prior to his move to Florida International University (FIU) in July 2014, Dr. Marcus Cooke led of one of the foremost groups in the UK undertaking oxidative stress research, specifically studying DNA damage. This expertise is now firmly established at FIU. He is internationally recognised as a leader in demonstrating the source and significance of urinary 8-oxo-7,8-dihydro-2’-deoxyguanosine, a non-invasive biomarker of oxidative stress. He continues to chair the EU-funded, European Standards Committee on urinary (DNA) lesion analysis, an international project comprising more than 30 labs/SMEs, the goal of which is the validation of urinary DNA lesions as important biomarkers for future large-scale human studies.
He is a member of the following EU-funded COST Actions: EU-ROS (€ 64 million COST Action; 2012 – 2016) and hCOMET. From a total of 119 publications, 85 are original primary data articles, the majority of which are in the top three journals for the field. He is an inventor on one granted patent, two pending patents and a recipient of two license agreements, as well as a co-editor of one book and a guest editor of a special DNA damage edition of Free Radical Research (2012). He is also an editorial board member for Scientific Reports and Biomarkers and a peer reviewer for more than 75 journals and major funding bodies, such as World Cancer Research Fund; EPSRC; Italian Telethon Foundation; Wellcome Trust, BBSRC; Medical Research Council; International Union Against Cancer; Agence Nationale de la Recherche; National Cancer Institute, and National Institute of Environmental Health Sciences.
Oxidative Stress Group laboratory studies the formation and repair of oxidatively damaged nuclear and mitochondrial DNA: from basic mechanisms to translational application of validated biomarkers. We have a particular interest in the nucleotide precursor pool as a target for genotoxins, together with genome-wide analysis of DNA damage, repair and chromatin remodelling. We actively promote the commercial exploitation of research findings and expose this concept to all who work in the lab.
- Fellow of the Royal College of Pathologists (FRCPath)
- University of Leicester, MSc, Ph.D.
- Liverpool Polytechnic, BSc (Hons)
Links to publications can be found here:
Shih, Y-M., Cooke, MS., Pan, C-H., Chao, M-R. and Hu, C-W. (2019) Clinical relevance of guanine-derived urinary biomarkers of oxidative stress, determined by LC-MS/MS. Redox Biology. 20, 556-565.
Olinski, R., Gackowski, D. and Cooke, MS. (2018) Endogenously generated DNA nucleobase modifications: source, and significance as possible biomarkers of malignant transformation risk, and role in anticancer therapy. BBA. Reviews on Cancer 1869, 29-41.
Delinassios, G., Karbaschi, M., Cooke, MS*. and Young, AR*. (2018) Vitamin E inhibits the UVAI induction of “light” and “dark” cyclobutane pyrimidine dimers, and oxidatively generated DNA damage, in keratinocytes. Scientific Reports 8, 423, DOI:10.1038/s41598-017-18924-4. *Co-corresponding authors.
Kim, G., Karbaschi, M., Cooke, MS. and Gaitas, A. (2018) Evaluation of three light based whole blood pathogen inactivation methods enabled by a recirculating flow system. Photochemistry and Photobiology. 94, 744-751. DOI: 10.1111/php.12899
Cooke, MS. (2018) Mycosporine-like amino acids: does Nature make a better sunscreen? (Invited commentary.) British Journal of Dermatology 178, 1239.
Hu, C-W., Cooke, MS., Chang, Y-J. and Chao, M-R. Direct-acting DNA ethylating agents in cigarette smoke primarily originate by transfer from the tobacco itself, not combustion. Journal of Hazardous Materials. 358, 397-404. DOI: 10.1016/j.jhazmat.2018.07.011
Chang, Y-J., Cooke, MS., Hu, C-W. and Chao, M-R. (2018) Novel approach to integrated DNA adductomics for the assessment of in vitro and in vivo environmental exposures. Archives of Toxicology. 92, 2665-2680.
Chao, M-R*., Cooke, MS*., Chang, Y-J., Pan, C-H, Liu, H-H. Yang, H-J., Chiang, Y-C. and Hu, C-W. (2018) Children are particularly vulnerable to environmental tobacco smoke exposure: evidence from biomarkers of tobacco-specific nitrosamines, and oxidative stress. Environment International. 120, 238-245. *Contributed equally.
Shih, BB., Farrar, MD., Cooke, MS., Osman, J., Langton, AK., Kift, R., Webb, AR., Berry, JL., Watson, REB., Vail, A., de Gruijl, FR. and Rhodes, LE. Sub-sunburn produces vitamin D and DNA damage equivalently but with absent to strong damage gradients in human skin types I-VI. Journal of Investigative Dermatology (in press). doi: 10.1016/j.jid.2018.04.015.
Egea, J., Fabregat, I., Frapart, Y.M., Ghezzi, P., Görlach, A., Kietzmann, T., Kubaichuk, K., Knaus, U. G., Lopez, M.G., Olaso-Gonzalez, G., Petry, A., Schulz, R., Vina, J., Winyard, P., Abbas,K., Ademowo, S., Afonso, C.B., Andreadou, L., Antelmann, H.,Antunes, F., Aslan, M., Bachschmid, M.M., Barbosa, R. M., Belousov, V., Berndt, C., Bernlohr, D., Bertrán, E., Bindoli, A., Bottari, S.P., Brito, P.M., Carrara, G., Casas, A.I., Chatzi, A., Chondrogianni, N., Conrad, M., Cooke, M.S., Costa. J.G., 34, Cuadrado, A., Dang, P.M., Smet, B.D., Debelec–Butuner, B., Dia, I., Dunn, J.D., Edson, A.J, Assar, M.E., Benna, J.E., Ferdinandy, P., Fernandes, A.S., Fladmark, K.E., Förstermann, U., Giniatullin, R., Giricz, Z., Görbe, A., Griffiths, H., Hampl, V., Herget, J., Hernansanz-Agustín, P., Hillion, M., Huang, J., Ilikay, S., Jansen-Dürr, P., Jaquet, V., Joles, J.A., Kalyanaraman, B., Kaminskyy, D., Karbaschi, M., Kleanthous, M., Klotz, L.O., Korac, B.,Korkmaz, K.S., Koziel, R., Kračun, D., Krause, K.H., Křen, V., Krieg, T., Laranjinha, J., Lazou, A., Li, H., Martínez-Ruiz, A., Matsui, R., McBean, G.J., Meredith, S.P., Messens, J., Miguel, V., Mikhed, Y., Milisav, I., Milković, L., Miranda-Vizuete, A.,Mojović, M., Monsalve, M., Mouthuy, P.A., Mulvey, J., Münzel, T., Muzykantov, V., Nguyen, I.T.N., Oliveira, N.G., Palmeira, C.M., Papaevgeniou, N., Pavićević, A., Pedre, B., Peyrot, F., Phylactides, M., Pircalabioru, G.G., Pitt, A.R., Poulsen, H.E., Prieto, I., Rigobello, M.P., Robledinos-Antón, N., Rodríguez-Mañas, L., Rolo, A.P., Rousset, F., Ruskovska, T.,Saraiva, N., Sasson, S., Schröder, K., Semen, K., Seredenina, T., Shakirzyanova, A., Smith, G.L., Soldati, T., Sousa, B.C., Spickett, C.M., Stancic, A., Stasia, M.J., Steinbrenner, H., Stepanić, V., Steven, S., Tokatlidis, K., Tuncay, E., Turan, B., Ursini, F., Vacek, J., Vajnerova, O., Valentová, K., Varisli, L., Veal, E.A., Weber, A., Yalçın, A.S., Yelisyeyeva, O., Žarković, N., Zatloukalová, M., Zielonka, J., Touyz, R.M., Papapetropoulos, A., Grune, T., Lamas, S.,Schmidt, H.H., Lisa, F.D., and Daiber, A. (2017) Position paper by the EU-ROS consortium on RONS in health and disease: European Contribution to the study of ROS: A Summary of the Findings and Prospects for the Future from the COST Action BM1203 (EU-ROS). Redox Biology. 13, 94-162.
Rossner, Jr., P., Orhan, H., Santella, RM., Sakai, K., Koppen, G., Ambroz, A., Rossnerova, A., Sram, RJ., Ciganek, M., Neca, J. and Cooke, MS. (2016) Urinary 8-oxo-7,8-dihydro-2′-deoxyguanosine analysis by an improved ELISA: does assay standardization reduce inter-laboratory variability? Free Radic. Biol. Med. 95, 169-179.
Felton, SJ., Cooke, MS., Kift, R., Berry, JL., Webb, AR., Lam, PMW., de Gruijl, FR., Vail, A. and Rhodes, LE. (2016) Concurrent beneficial (vitamin D production) and hazardous (cutaneous DNA damage) impact of low level summer sunlight exposures. Br. J. Dermatol. 175, 1320-1328. DOI: 10.1111/bjd.14863.
Evans, MD., Misty, V., Singh, R., Gackowski, D., Różalskic, R., Siomek-Goreckac, A., Phillips, DH., Zuod, J., Mullenders, L., Pines, A., Nakabeppu, Y., Sakumi, K., Sekiguchi, M., Tsuzuki, T., Bignami, M., Oliński, R. and Cooke, MS. (2016) Nucleotide excision repair of oxidised genomic DNA is not the primary source of urinary 8-oxo-7,8-dihydro-2’-deoxyguanosine. Free Radic. Biol. Med. 99, 385-391.
Hu, C-W., Cooke, MS., Tsai, Y-H. and Chao, M-R. (2015) Comparison of 8-oxo-7,8-dihydroguanine and 8-oxo-7,8-dihydro-2′-deoxyguanosine concentrations in various human body fluids measured by isotope-dilution LC-MS/MS. Arch. Toxicol. 89, 201-210. DOI: 10.1007/s00204-014-1255-1
Karbaschi, M., Macip, S, Mistry, V., Abbas, HHK, Delinassios, GJ., Evans, MD., Young, AR. and Cooke, MS. (2015) Rescue of cells from apoptosis increases DNA repair in UVB exposed cells with implications for the DNA damage response. Toxicol. Res. 4, 725-738. DOI: 10.1039/c4tx00197d
Part of a special “New Talent” edition: http://pubs.rsc.org/en/journals/journalissues/tx?e=1#!issueid=tx004003&type=current&issnprint=2045-452x)